Advalab’s Fluorometer is designed with optical filters or monochromators for both excitation and emission wavelengths. They feature intuitive software and touchscreen controls for easy data acquisition and analysis. Their capability to detect low concentrations of fluorescent compounds with excellent precision. These units use USB, Bluetooth, or Wi-Fi to export results and integrate with lab systems. The Fluorometer detects trace biomolecules, pollutants, and dyes, essential for life sciences and research.
1. What is a Fluorometer?
A Fluorometer is an analytical instrument used to measure parameters of fluorescence, including intensity and wavelength. It is commonly used to detect and quantify trace amounts of substances in various samples. By exciting a sample with a specific wavelength of light, it measures the emitted fluorescence, providing valuable data. Fluorometers are widely used in fields such as biochemistry, environmental science, and clinical diagnostics.
2. What factors can affect the accuracy of Fluorometer readings?
Several factors can affect Fluorometer accuracy, including sample turbidity or color, which can scatter or absorb light. Quenching agents may reduce fluorescence intensity, and improper instrument calibration can lead to errors. Incorrect excitation/emission filter settings and background fluorescence can also interfere with accurate readings.
3. What’s the difference between a Fluorometer and a spectrophotometer?
A Fluorometer measures fluorescence (light emitted by a substance after excitation), while a spectrophotometer measures absorbance (how much light is absorbed). Fluorometers are typically more sensitive than spectrophotometers. Unlike spectrophotometers, which measure light passing through the sample, fluorometers detect emitted light, allowing for lower detection limits.