Automated Cell Counter ADACC-501

1. What is an Automated Cell Counter ADACC-501 and how does it work?

An Automated Cell Counter ADACC-501 is a laboratory instrument used to quickly and accurately count cells in a liquid sample. It uses imaging technology, electrical impedance, or flow cytometry to detect and analyze individual cells. These systems can also provide data on cell viability, size, and concentration. By automating the process, it eliminates the subjectivity and time required in manual counting using a hemocytometer.

2. What types of cells can be counted with an Automated Cell Counter ADACC-501?

Automated Cell Counter ADACC-501 is versatile and can handle a wide range of cell types including mammalian cells, yeast, algae, and some types of bacteria. It is particularly useful in tissue culture labs, bioprocessing, and clinical research. It comes with specialized settings or reagents for specific cell lines or applications. Viable and non-viable cells can also be distinguished using dye-based methods.

3. How accurate is an Automated Cell Counter ADACC-501 compared to manual counting?

Automated Cell Counter ADACC-501 is generally more accurate and consistent than manual methods, especially when counting large numbers of samples. It reduces human error, fatigue, and variability between users. Accuracy can still be influenced by factors such as sample preparation and debris in the sample. Most systems offer high reproducibility and are validated against manual hemocytometer counts.

4. Do I need to use stains or dyes with an Automated Cell Counter ADACC-501?

Stains or dyes are often used to differentiate between live and dead cells in Automated Cell Counter ADACC-501. Trypan Blue is one of the most commonly used dyes for viability assessment. It uses fluorescence-based stains for more advanced analysis. However, basic total cell counts can sometimes be done without any staining, depending on the device.

5. How do I prepare a sample for Automated Cell Counter ADACC-501?

Sample preparation typically involves creating a uniform cell suspension with the appropriate concentration. Clumping should be avoided, often by gently pipetting or using enzymatic treatment like trypsinization. Debris should be minimized as it can interfere with accurate counts. It requires dilution or the addition of specific reagents or dyes. Proper mixing and loading technique is essential to ensure accurate and reliable results.