| Capacity | 0.2 mL × 96-well, aluminum |
| Thermal Control System | Three independently controlled Peltier modules with peripheral edge heating |
| Temperature Control Accuracy | ±0.1°C |
| Block Temperature Precision | ≤±0.1°C |
| Temperature Uniformity | ≤±0.3°C at 55°C |
| Maximum Heating Rate | >5°C/s |
| Maximum Cooling Rate | >5°C/s |
| Average Ramp Rate | >2°C/s |
| Ramp Rate Adjustment Range | 0.1 to 4.0°C/s |
| Block Temperature Range | 4°C to 99°C |
| Heated Lid Temperature Range | 30°C to 110°C, ON or OFF switchable |
| Block Control Mode | Block-based or tube-based temperature control |
| Gradient Temperature Range | 30°C to 99°C; gradient span: 0.1°C to 42°C |
| Temperature Touchdown Function | 0.1°C to 9.9°C per cycle |
| Time Touchdown Function | 1 second to 9 minutes 59 seconds per cycle |
| Smart Ramp Rate | Yes |
| Non-Specific Amplification Suppression | Yes |
| Real-Time Temperature Graph | Yes |
| Auto-Adaptive Heated Lid | Yes |
| Display Interface | 7 |
| Maximum PCR Steps per Program | 30 |
| Program Storage Capacity | Up to 1296 individual protocols |
| Power Failure Recovery | Yes |
| Post-Run Soak Mode | Yes, holds samples at 8°C automatically |
| Remaining Time Display | Yes |
| On-the-Fly Program Editing | Yes |
| Remote Service Support | Yes, via We Chat platform |
| Certification | CE marked (conforms to EU standards) |
| Applicable Consumables | Microplates (non-skirted/semi-skirted), 0.2 mL tubes (single/8-strip/12-strip), all tube shapes |
| Power Supply | 100–240V AC, 50/60 Hz, 600 VA |
| Product Dimensions (L × W × H) | 427 × 288 × 227 mm |
| Shipping Dimensions (L × W × H) | 540 × 450 × 385 mm |
| Net Weight | 9.7 kg |
| Gross Weight | 14.5 kg |
This Gradient Thermal Cycler ADGTC-505 is used in molecular biology laboratories for precise DNA amplification through PCR, ensuring accurate results in diagnostics, research, and genetic analysis.
1. What is the capacity of the Gradient Thermal Cycler ADGTC-505?
The Gradient Thermal Cycler ADGTC-505 features a dual-block system accommodating 0.2 mL × 96-well tubes. Its aluminum construction enhances thermal uniformity and transfer. This layout supports high-throughput PCR workflows. The block’s design enables consistent heating across all wells. It is suitable for laboratories handling large sample volumes.
2. How accurate is Gradient Thermal Cycler ADGTC-505 temperature control?
This Gradient Thermal Cycler ADGTC-505 delivers temperature regulation accuracy of ±0.1°C. The integrated Peltier modules and edge heating ensure stability. It maintains consistent block precision, minimizing variability. Such accuracy is essential for reliable DNA amplification. The system is optimized for sensitive thermal cycling conditions.
3. What user interface does the Gradient Thermal Cycler ADGTC-505 offer?
The Gradient Thermal Cycler ADGTC-505 is equipped with a 7" full-color WVGA touchscreen. It provides intuitive navigation through protocols and settings. LED backlighting ensures visibility in various lab environments. Users can operate and monitor in real-time. The interface supports multiple program customizations with ease.
4. What is the benefit of having an adjustable ramp rate in the Gradient Thermal Cycler ADGTC-505?
An adjustable ramp rate allows precise control over temperature transitions between PCR steps. This is especially useful when optimizing protocols for different enzymes or templates. Slower ramp rates can enhance specificity, while faster ones reduce overall runtime. Researchers gain greater flexibility in designing experiments. It’s an essential feature for advanced thermal cycling.
5. What advanced features does Gradient Thermal Cycler ADGTC-505 include?
The Gradient Thermal Cycler ADGTC-505 offers gradient temperature synchronization for uniform results. An adaptive hot lid fits various tube formats without manual adjustment. Ramp rate is customizable for optimized reactions. Non-specific amplification is intelligently controlled to improve accuracy. The design prevents evaporation and supports reproducibility.
6. What is Gradient Thermal Cycler?
Gradient Thermal Cycler is a technique that uses a thermal cycler with a temperature gradient across the heating block. This allows users to test multiple annealing temperatures in a single run, helping to quickly identify the optimal temperature for primer binding. It is especially useful during primer validation and assay development.
7. Why is Gradient Thermal Cycler used?
Gradient PCR is used to optimize PCR conditions, especially the annealing temperature, which is critical for specificity and efficiency. It helps reduce trial-and-error by allowing multiple conditions to be tested simultaneously. This improves accuracy while saving time and reagents during PCR setup.
8. What are the benefits of Gradient Thermal Cycler?
Gradient Thermal Cycler offers several benefits, particularly during the optimization phase of PCR. It saves time by allowing multiple annealing temperatures to be tested in a single run, eliminating the need for separate experiments. This approach also reduces reagent use and overall costs. Gradient PCR improves efficiency and specificity by quickly identifying the optimal annealing temperature.
| Capacity |
96×0.2 ml (PCR plate without/semi skirt) 12×8×0.2 ml (strips) 8×12×0.2 ml (strips) 0.2ml tubes (height 20 to 23mm) |
| Maximum Heating Rate | 5℃/s |
| Maximum Cooling Rate | 4.5℃/s |
| Capacity | 0.2 mL × 384-well, aluminum |
| Thermal Control System | Three independently controlled Peltier modules with peripheral edge heating |
| Temperature Control Accuracy | ±0.1°C |
| Tubes Capacity |
0.2 ml microtubes 8 × 0.2 ml tube strips 96 × 0.2 ml PCR plate (no/semi-skirted) |
| Capacity | 96 × 0.2 ml |
| Maximum Heating Up Rate | 6℃/s |
| Capacity | 96 x 0.2ml (0.2ml PCR tubes, 8-tube strips) |
| Temperature Setting Range | 4℃ to 105℃ |
| Gradient Temperature Range | 30 to 105℃ |
| Capacity: | 2 × 0.2 mL × 48-wells, aluminum |
| Thermal Control System : | Three independently controlled Peltier modules with peripheral edge heating |
| Temperature Control Accuracy : | ±0.1°C |
| Capacity: | 2 × 0.2 mL × 48-wells, aluminum |
| Thermal Control System: | Peltier |
| Temperature Control Accuracy : | ±0.1°C |
| Capacity: | 32 x 0.2ml tubes, 4 x 8 tube strips |
| Temperature Setting Range: | 0℃ to 100℃ |
| Maximum Heating Time: | 4℃/sec |