Vertical Autoclave ADVA-523

1. What is a vertical autoclave and how does it work?

A vertical autoclave is a cylindrical sterilization device designed to use pressurized steam to eliminate bacteria, viruses, fungi, and spores from equipment and materials. It works by heating water to produce steam, which builds pressure within a sealed chamber. The high temperature and pressure effectively sterilize items placed inside. These autoclaves are loaded from the top, making them space-efficient for small labs and clinics. They're ideal for laboratories, hospitals, and research institutions.

2. What are the typical applications of a vertical autoclave?

Vertical autoclave is widely used for sterilizing glassware, media, instruments, and laboratory waste. Common applications include microbiology, pharmaceutical production, food testing labs, and hospital surgical units. It is essential for preventing contamination and ensuring safe reuse of lab and clinical equipment. It is also used to prepare culture media. Its versatility makes them a go-to sterilization tool across scientific fields.

3. What materials can be sterilized in a vertical autoclave?

Vertical autoclave can sterilize glassware, metal instruments, fabric, rubber, and certain types of plastic. Liquids such as culture media or laboratory reagents can also be sterilized if the autoclave has a liquid cycle. Items must be autoclavable, meaning they can withstand high temperature and pressure. Materials with low melting points or sealed containers that may explode should be avoided. Always follow manufacturer guidelines for safe sterilization.

4. What are the standard temperature and pressure settings for vertical autoclave sterilization?

The most common sterilization settings are 121°C at 15 psi pressure for 15–30 minutes. Some models allow higher settings, such as 134°C at 30 psi, for faster sterilization. The time, temperature, and pressure can vary based on the load type and volume. Liquid cycles typically require lower pressure and longer holding times. Consistent monitoring ensures effective sterilization and equipment safety.

5. How long does a typical sterilization cycle take in a vertical autoclave?

A typical cycle lasts between 30 to 60 minutes, including heating, sterilization, and cooling phases. The sterilization (holding) phase alone is usually 15–30 minutes at 121°C. Heavier or denser loads take longer to heat and cool. Liquid loads often require extended time to avoid boil-over. Cycle duration can be adjusted based on the load type.