Split Beam Spectrophotometer ADSBS-501 analyses samples in the visible to near-infrared range from 190 to 1100 nm. It provides enhanced wavelength accuracy and photometric reliability for high-precision results. This unit maintains consistent illumination, making it suitable for kinetic-based studies. The split-beam design corrects light fluctuations by real-time beam comparison. Our Split Beam Spectrophotometer is used in pharmaceuticals, food analysis, and academic research for accurate absorbance.
| Optical System | Split Beam, Grating 1200 lines/mm |
| Wavelength Range | 190 to 1100 nm |
| Spectral Bandwidth | 2 nm |
| Wavelength Accuracy | ± 0.1nm, 656.1nm, ± 0.3nm all |
| Wavelength Repeatability | <=0.1nm |
| Photometric Accuracy | ± 0.2%T (0 to 100 %T), ± 0.0005A (0 to 0.5A), ± 0.0008A (0.5 to 1A) |
| Photometric Repeatability | ≤0.15%T (0 to 100%T), 0.0004A(0 to 0.5A), 0.0006A (0.5 to 1A) |
| Photometric Range | 0-400%T, -4 to 4A |
| Resolution | 0.00001Abs |
| Stray Light | <=0.03%T, 220 nm, 360 nm |
| Stability | ± 0.0001A |
| Baseline Flatness | ± 0.0004A |
| Noise | 0.0005A, 500 nm |
| Work Mode | T, A, C, E |
| Scanning Speed | High, medium, low (Max. 6000 nm/min) |
| Display | 11-inch Touch screen |
| Light Source | Deuterium and tungsten lamp |
| Detector | Imported silicon photodiode |
| Cuvette Holder | 10mm Single hole cell holder |
| Output | Cloud connectivity/Email/SMS/Bluetooth/WiFi |
| Power | AC 110 to 220 V 50-60Hz |
| Dimensions (L×B×H) | 810×660×390 mm |
| Gross Weight | 27 kg |
Our Split Beam Spectrophotometer ADSBS-501 is used in clinical diagnostics, quality control, and environmental monitoring for accurate and reliable absorbance-based measurements.
Accessory Quantity 10 mm glass cuvette 4 Pc 10 mm quartz cuvette (UV) 2 Pc Power cord 1 Pc User's Manual 1 Pc
1. What is the principle of the Split Beam Spectrophotometer ADSBS-501?
The Split Beam Spectrophotometer ADSBS-501 works by splitting the light beam into two paths—one for the sample and one for the reference. This design allows simultaneous measurement and correction of light source fluctuations. It ensures more accurate absorbance readings over time. The reference beam continuously monitors changes in lamp intensity. This results in improved stability and precision in quantitative analysis.
2. What are the main features of the Split Beam Spectrophotometer ADSBS-501?
The Split Beam Spectrophotometer ADSBS-501 offers a split beam design, high photometric accuracy, wide wavelength range, and user-friendly interface. It provides automatic baseline correction and data processing. Its real-time reference compensation reduces measurement errors. It typically includes a graphical LCD display and USB data output. The instrument is ideal for precise UV-Vis absorbance analysis.
3. How is the Split Beam Spectrophotometer ADSBS-501 different from single beam spectrophotometers?
Unlike single beam models, the Split Beam Spectrophotometer ADSBS-501 simultaneously measures the sample and reference beams. This compensates for fluctuations in lamp output and environmental factors. As a result, it offers better accuracy and reproducibility. It is especially useful in long-duration experiments or for samples with subtle absorbance differences. The split beam system enhances reliability in routine and research applications.
4. What are the applications of the Split Beam Spectrophotometer ADSBS-501 in various fields?
The Split Beam Spectrophotometer ADSBS-501 is used in pharmaceuticals, life sciences, environmental testing, food quality control, and academic research. It accurately quantifies DNA/RNA, proteins, drugs, and chemicals. In environmental labs, it's applied for analyzing water and air pollutants. Food labs use it to check additives, colors, and nutrient levels. Its precision and stability make it suitable for a wide range of analytical tasks.
5. How do you calibrate and maintain the Split Beam Spectrophotometer ADSBS-501?
Calibration involves using standard reference materials and following the manufacturer's guidelines. Regular wavelength accuracy and photometric accuracy checks are essential. The optical surfaces should be kept clean and the instrument covered when not in use. Periodic software updates and diagnostics help maintain performance. Scheduled maintenance ensures long-term accuracy and device reliability.
| Wavelength Range: | 325 to 1050 nm |
| Wavelength Accuracy: | ± 2 nm |
| Wavelength Reproducibility: | 1 nm |
| Wavelength Range: | 320 to 1050 nm |
| Wavelength Accuracy: | ± 2 nm |
| Wavelength Reproducibility: | 1 nm |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 1 nm |
| Display: | 7-inch Colour Screen |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Display: | 7-inch Colour Screen |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 0.1/0.2/0.5/1/2/4 /5 nm |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 1 nm |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 2 nm |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 1 nm |
| Wavelength Range: | 190 to 1100 nm |
| Wavelength Accuracy: | ± 0.3 nm |
| Wavelength Repeatability: | ≤ 0.2 nm |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 1 nm |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 0.1/0.2/0.5/1/ 2/4/5 nm |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 2 nm |
| Display: | 7-inch Colour Screen |
| Optical System: | Double Beam, Grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Wavelength Range: | 190 to 1100 nm |
| Wavelength Accuracy: | ± 0.3 nm |
| Wavelength Repeatability: | ≤ 0.2 nm |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 0.5/1/2/4/5 nm |
| Optical System: | Double Beam, Grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 2 nm |
| Display: | 10.1 TFT coloured capacitive touch screen |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 0.5/1/2/4/5 nm |
| Optical System: | Double Beam, Grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 0.5/1/2/4/5 nm |
| Display: | 7-inch Colour Screen |
| Optical System: | Double Beam, Grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Display: | 7-inch Colour Screen |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Display: | 7-inch Colour Screen |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Optical System: | Single beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 2 nm |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 2 nm |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 1 nm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 0.1 /0.2/ 0.5/ 1/ 2/ 5 nm |
| Working Modes: | Transmittance, Absorbance, Concentration, Energy |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 0.5/1/2/4/5 nm |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 1 nm |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 2 nm |
| Display: | 7-inch Colour screen |
| Optical System: | Double beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Wavelength Range: | 195 to 1000 nm |
| Wavelength Accuracy: | ± 2 nm |
| Transmittance Range: | 0 to 199.9 |
| Wavelength Range: | 340 to 1000 nm |
| Wavelength Accuracy: | ± 3 nm |
| Transmittance Range: | 0 to 199.9 |
| Wavelength Range: | 325 to 1000 nm |
| Wavelength Accuracy: | ± 2 nm |
| Transmittance Range: | 0 to 199.9 |
| Optical System: | Single beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 1 nm |
| Optical System: | Single Beam, Grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 1 nm |
| Optical System: | Single beam |
| Monochromator: | C-T Monochromator, blazed holographic grating 1200 lines/nm |
| Wavelength Range: | 190 to 1100 nm |
| Optical System: | Single Beam, Grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 2 nm |
| Optical System: | Single beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 0.1/0.2/0.5/1/ 2/4/5 nm |
| Optical System: | Single beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 2 nm |
| Optical System: | Single beam |
| Monochromator: | C-T Monochromator, blazed holographic grating 1200 lines/nm |
| Wavelength Range: | 320 to 1100 nm |
| Optical System: | Single beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 2 nm |
| Optical System: | Single beam |
| Monochromator: | C-T Monochromator, blazed holographic grating 1200 lines/nm |
| Wavelength Range: | 190 to 1020 nm |
| Optical System: | Single beam |
| Monochromator: | C-T Monochromator, blazed holographic grating 1200 lines/nm |
| Wavelength Range: | 320 to 1100 nm |
| Optical System: | Single beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 0.5/1/2/4/5nm |
| Sample Volume: | 0.5 to 2.0 μL |
| Nucleic Acid Detection Range: | 2 to 15000 ng/μL (dsDNA) <br/> 1.32 to 9900 ng/μL (ss DNA) <br/> 1.6 to12000ng/μL (RNA) |
| Protein Detection Range: | 0.04 to 750 mg/mL (A280) <br/> 0.06 to 1119 mg/mL (BSA) <br/> 0.03 to 547 mg/mL (IgG) <br/> 0.015 to 284 mg/mL (lysozyme) |
| Optical System: | Single beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 0.5/1/2/4/5 nm |
| Optical System: | Single beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 2 nm |
| Optical System: | Single Beam, Grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 1 nm |
| Sample Volume: | 0.5μl to 2μl (recommended to use 2μl) |
| Nucleic Acid Detection Range: | 2 to 37500ng /μL (dsDNA) <br/> 1.32 to 24750 ng/μL (ss DNA) <br/> 1.6 to 30000ng/μL (RNA) |
| Protein Detection Range: | 0.04 to 750mg/mL (A280) <br/> 0.06 to 1119mg/mL (BSA) <br/> 0.03 to 547mg/mL (IgG) <br/> 0.015 to 284mg/mL (lysozyme) |
| Optical System: | Single beam |
| Monochromator: | C-T Monochromator, blazed holographic grating 1200 lines/nm |
| Wavelength Range: | 190 to 1020 nm |
| Optical System: | Single Beam, Grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 0.5/1/2/4/5nm |
| Optical System: | Single beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 2 nm |
| Optical System: | Single Beam, Grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 0.1/0.2/0.5/1/2/4 |
| Optical System: | Single beam, grating 1200 lines/mm |
| Wavelength Range: | 320 to 1100 nm |
| Spectral Bandwidth: | 2 nm |
| Optical System: | Single beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 1 nm |
| Optical System: | Single beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 2 nm |
| Optical System: | Single beam |
| Monochromator: | C-T Monochromator, blazed holographic grating 1200 lines/nm |
| Wavelength Range: | 320 to 1020 nm |
| Optical System: | Single beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 1 nm |
| Optical System: | Single Beam, Grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 2 nm |
| Optical System: | Single beam, grating 1200 lines/mm |
| Wavelength Range: | 190 to 1100 nm |
| Spectral Bandwidth: | 1 nm |